| 12-Aug-00 | Plymouth Argyle | Away | League | 1-0 | Griffiths | 5649 | |
| 19-Aug-00 | Carlisle United | Home | League | 1-0 | Lockwood | 4320 | |
| 22-Aug-00 | Reading | Home | LC r1-1 | 1-1 | Brkovic | 2316 | |
| 26-Aug-00 | Blackpool | Away | League | 2-2 | Garcia (2) | 4816 | |
| 28-Aug-00 | Exeter City | Home | League | 2-1 | Lockwood, McGhee | 4113 | |
| 2-Sep-00 | Halifax Town | Away | League | 2-2 | Smith, Griffiths | 1592 | |
| 5-Sep-00 | Reading | Away | LC r1-2 | 2-0 | Brkovic, Christie | 4337 | |
| 9-Sep-00 | Hull City | Home | League | 2-2 | Griffiths, Christie | 5177 | |
| 12-Sep-00 | Scunthorpe United | Home | League | 1-1 | Lockwood | 3608 | |
| 16-Sep-00 | Kidderminster Harriers | Away | League | 1-2 | Christie | 3365 | |
| 20-Sep-00 | Newcastle United | Away | LC r2-1 | 0-2 | | 37284 | |
| 23-Sep-00 | Lincoln City | Home | League | 1-0 | Griffiths | 3889 | |
| 26-Sep-00 | Newcastle United | Home | LC r2-2 | 1-1 | Watts | 9522 | |
| 30-Sep-00 | Brighton & Hove Albion | Away | League | 0-2 | | 6731 | |
| 8-Oct-00 | Torquay United | Away | League | 2-1 | Griffiths, Garcia | 1925 | |
| 14-Oct-00 | Cardiff City | Home | League | 2-1 | Lockwood, Smith | 4649 | |
| 17-Oct-00 | Shrewsbury Town | Home | League | 2-0 | Walschaerts, Watts | 3554 | |
| 21-Oct-00 | York City | Away | League | 1-1 | Garcia | 2744 | |
| 24-Oct-00 | Barnet | Home | League | 3-1 | Lockwood, Walschaerts, Griffiths | 4707 | |
| 28-Oct-00 | Hartlepool United | Away | League | 1-2 | Watts | 2133 | |
| 4-Nov-00 | Mansfield Town | Home | League | 2-1 | Lockwood (pen), Griffiths | 4165 | |
| 11-Nov-00 | Cheltenham Town | Away | League | 1-1 | McGhee | 3161 | |
| 18-Nov-00 | Barrow | Away | FAC r1 | 2-0 | Griffiths, Watts | 3608 | |
| 25-Nov-00 | Macclesfield Town | Home | League | 2-1 | Brkovic, Watts | 4013 | |
| 2-Dec-00 | Chesterfield | Away | League | 1-4 | Watts | 4985 | |
| 9-Dec-00 | Northwich Victoria | Away | FAC r2 | 3-3 | Griffiths (2), Tate | 24703 | |
| 16-Dec-00 | Darlington | Home | League | 1-0 | Houghton | 4114 | |
| 20-Dec-00 | Northwich Victoria | Home | FAC r2r | 3-2 | Griffiths, Simpson (og), Houghton | 4028 | |
| 23-Dec-00 | Rochdale | Home | League | 1-1 | Walschaerts | 4483 | |
| 26-Dec-00 | Southend United | Away | League | 1-0 | Griffiths | 9595 | |
| 6-Jan-01 | Tottenham Hotspur | Home | FAC r3 | 0-1 | | 12336 | |
| 9-Jan-01 | Wycombe Wanderers | Home | LDV s.r2 | 0-2 | | 946 | |
| 13-Jan-01 | Exeter City | Away | League | 3-2 | Griffiths (2), Tate | 3044 | |
| 20-Jan-01 | Southend United | Home | League | 0-2 | | 6859 | |
| 28-Jan-01 | Rochdale | Away | League | 1-3 | Smith | 3662 | |
| 3-Feb-01 | Halifax Town | Home | League | 3-0 | Brkovic, Watts, Pinamonte | 3849 | |
| 10-Feb-01 | Hull City | Away | League | 0-1 | | 8782 | |
| 17-Feb-01 | Kidderminster Harriers | Home | League | 0-0 | | 4132 | |
| 20-Feb-01 | Scunthorpe United | Away | League | 1-1 | Griffiths | 2523 | |
| 24-Feb-01 | Lincoln City | Away | League | 3-2 | Brkovic, Watts, Pinamonte | 3027 | |
| 3-Mar-01 | Brighton & Hove Albion | Home | League | 0-2 | | 7958 | |
| 6-Mar-01 | Cardiff City | Away | League | 1-1 | Opinel | 9022 | |
| 10-Mar-01 | Torquay United | Home | League | 0-2 | | 4390 | |
| 13-Mar-01 | Carlisle United | Away | League | 0-1 | | 2610 | |
| 17-Mar-01 | Shrewsbury Town | Away | League | 1-1 | McLean | 2740 | |
| 20-Mar-01 | Blackpool | Home | League | 1-0 | Lockwood | 4086 | |
| 24-Mar-01 | York City | Home | League | 1-1 | Griffiths | 4085 | |
| 31-Mar-01 | Darlington | Away | League | 1-1 | Smith | 3189 | |
| 7-Apr-01 | Chesterfield | Home | League | 2-0 | McGhee, Griffiths | 4793 | |
| 10-Apr-01 | Plymouth Argyle | Home | League | 1-1 | Watts | 4520 | |
| 14-Apr-01 | Barnet | Away | League | 2-1 | Tate (2) | 3759 | |
| 16-Apr-01 | Hartlepool United | Home | League | 3-1 | Smith, Griffiths, Watts | 5359 | |
| 21-Apr-01 | Mansfield Town | Away | League | 0-2 | | 3356 | |
| 28-Apr-01 | Cheltenham Town | Home | League | 0-0 | | 5632 | |
| 5-May-01 | Macclesfield Town | Away | League | 2-0 | Ibehre (2) | 2522 | |
| 13-May-01 | Hull City | Away | PO sf-1 | 0-1 | | 13310 | |
| 16-May-01 | Hull City | Home | PO sf-2 | 2-0 | Lockwood, Watts | 9419 | |
| 26-May-01 | Blackpool | Millenium Stadium | PO fn | 2-4 | Tate, Houghton | 23600 | |